This study was aimed to assess the appropriateness of wastewater to irrigate soft and hard wheat crops. to evaluate growth and productivity of wheat crops. The investigation was conducted during the Agricultural season 2021-2022, at Zawia, Libya. The experiment utilized four different water regimes, including: (S1) Irrigation with 100% fresh water (control), (S2): Irrigated with 50% wastewater and 50% fresh water, (S3): Irrigated with 75% wastewater and 25% fresh water, (S4): irrigated with 100% wastewater. Wheat seeds were sowed in November 2021 in plastic pots and harvested at the start beginning of April 2022. The results showed that using wastewater significantly increased growth parameters, in terms of plant height, wastewater supplemented with (S4) resulted in the highest growth (46.5 cm/plant) in hard wheat and 45.12 cm/plant in soft wheat plants. Higher shoot fresh weights (5.5 g/plant), root fresh weights (3.28 g/plant), and root fresh weights (13.12 and 10.22 g/plant) for hard wheat and soft wheat were achieved, respectively, when wastewater was utilized in isolation as opposed to fresh water. In terms of shoot dry weight, irrigation with (S4) resulted in a yield of 2.00 g/plant and 1.42 g/plant, whereas plants irrigated with fresh water produced 0.92 g/plant and 0.82 g/plant, for soft wheat and soft wheat consecutively. The Sodium Adsorption Ratio (SAR) calculated for the study samples is within the low limits for sodium, which ranges from (0-10), as it was in this study from (1.78 to 4.49), all of which are much smaller than the safe value. and without any damage. This means that all water samples analyzed can be used for irrigation. RSC values ranged from-3 to-110 meq/L, all samples are located at the appropriate limits. SAR, RSC of the samples of water indicate that most of the water has no risk of irrigation. The study determined that the utilization of treated wastewater in the irrigation of agricultural crops, while being monitored, lacks economic viability.

A B S T R A C T

The current study aimed to the production and partial purification of a cold-active lipase by some fungi isolated from the olive oil processing wastes in Al-Gabal Al-Gharby, Libya. 31 fungal species from 12 genera were isolated. F. solani was the most prevalent comprising 94% of total Fusarium and 28.7% of total fungi, 102 fungal isolates were tested for their lipolytic activity on lipase production agar medium at 10 and 20°C. The most active isolates were Alternaria (2 isolates), Fusarium, and Penicillium (1isolate for each one). Molecular identification of the most active four isolates was carried out by their sequencing (ITS). The four powerful fungal strains' production of cold-active lipase was maximized by optimizing some nutritional and environmental factors. F. solani AUMC 16063 was able to produce the maximum amount of lipase activity (46.66U/mL/min) with specific activity (202.8U/mg), utilizing ammonium sulphate as a nitrogen source after 8 days of incubation at pH 3.0 and 15°C. However, at same condition after 6 days when yeast extract was employed as a nitrogen source, the generated cold-active lipase displayed the highest specific activity of (1550U/mg) and lipase activity (36.74U/ml/min). This is the first study in which the production, partial purification, maximized and characterization of a cold-active lipase enzyme by Fusarium solani.

Keywords: cold active enzymes, Lipase, Fusarium solani, lipolytic activity, specific activity.

The current study aimed to the production and partial purification of a cold-active lipase by som fungi isolated from the olive oil processing wastes in Al-Gabal Al-Gharby, Libya. 31 fungal species from 12 genera were isolated. F. solani was the most prevalent comprising 94% of total Fusarium and 28.7% of total fungi, 102 fungal isolates were tested for their lipolytic activity on lipase production agar medium at 10 and 20°C. The most active isolates were Alternaria (2 isolates), Fusarium, and Penicillium (1isolate for each one). Molecular identification of the most active four isolates was carried out by their sequencing (ITS). The four powerful fungal strains' production of cold-active lipase was maximized by optimizing some nutritional and environmental factors. F. solani AUMC 16063 was able to produce the maximum amount of lipase activity (46.66U/mL/min) with specific activity (202.8U/mg), utilizing ammonium sulphate as a nitrogen source after 8 days of incubation at pH 3.0 and 15°C. However, at same condition after 6 days when yeast extract was employed as a nitrogen source, the generated cold-active lipase displayed the highest specific activity of (1550U/mg) and lipase activity (36.74U/ml/min). This is the first study in which the production, partial purification, maximized and characterization of a cold-active lipase enzyme by Fusarium solani. Keywords: cold active enzymes, Lipase, Fusarium solani, lipolytic activity, specific activity. 

Abstract: This study aims to analyzing demographic data of patients, isolate and identify Candida species, which causes vaginal infections, and a study of its prevalence among pregnant women in Sorman city, Libya.210 specimens collected from patients admitted to the Maternity Care Center in the combined clinic. carried immediately to the Microbiology Laboratory in the National Cancer Institute, Subrata, Libya for direct microscopy, culturing, and characterization. Each participant was given an interview questionnaire and asked about their age, educational level, employment position, and history of recurrent vaginal yeast infection. Chronic diseases were also listed on the data collecting form. Identification of Candida species using Chrome agar: A total of 100 isolates have been recovered in this study, of which 72isolates were obtained as pure cultures on Chrome agar medium. According to their color on Chrome agar, these 72 colonies were categorized to 5 main species namely Candida albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis. Genotypic identification of Candida species in this investigation was validated by the ITS tree. Eight strains from this investigation were found in the Candida albicans clade, which had a high bootstrap value of 99 percent ML/99 percent MP. These were therefore identified as Candida albicans., Within the Candida glabrata clade, three isolates were grouped together, demonstrating a strong support value of 99% ML/99%MP. These strains were recognized as belonging to the C. glabrata species, while one isolate was recognized as belonging to the C. tropicalis species, with a high support value of 99% ML/99%MP.

 Keywords: VVC infections, candidemia patients, genotypic identification, and Candida albicans

 Food poisoning caused by bacterial agents is a worldwide problem, usually accompanied by unpleasant symptoms and may be sever leading to death. Natural compounds from marine algae namely flavonoids may play a role in the remedy of this condition. Aim: This research aims to assess the potency of flavonoids extracted from Enteromorpha intestinalis and Caulerpa prolifera as antibacterial agents. Methods: E. intestinalis was collected from Western Libyan Coast and C. prolifera was collected from Farwa Island. The antimicrobial activity and determination of minimum inhibitory concentration of algal flavonoid containing extracts was performed in vitro against some positive and negative Gram bacteria. Results: Crude extract containing flavonoids from E. intestinalis were more effective than C. prolifera extract against Staphylococcus aureus with antimicrobial essay (25-28+1 and 14.5-37.5+0.5-1.5), MIC (50 and 50-250 µg/mL), MBC (75 and 75-250 µg/mL). In Bacillus cereus, the antimicrobial assay (19-24.5+0.5-1.5: 24+1), MIC (50-250 + 100 µg/mL) and MBC (250 and 125 µg/mL). On the other hand, flavonoids containing extract from C. prolifera were more effective than E. intestinalis against Enterohemorrhagic Escherichia coli O157 EHEC O157 (25-28+1: 14-18.5+0.5-1.5), MIC (100-250:100-500 µg/mL) and MBC (150-250 and 250-500 µg/mL). Salmonella enterica qualitatively combat by flavonoid from E. intestinalis (13.5-14+0.5-1: 10.5-13.5+0.5-1.5), MIC (100-250: 250 µg/mL) and MBC (100-250: 250 µg/mL). Flavonoids from C. prolifera (4 strains: 2 strains) were effective against S. enterica. Crude flavonoids from both algae were not effective against Bacillus pumilus. Conclusion: Data from this study could conclude that flavonoid extracts from E. intestinalis and C. prolifera could be used against foodborne bacterial agents

Abstract: The current study was aimed for isolation, identification and preservation of mycobiota associated with the olive oil processing wastes (Fatura) collected from different cities in Al-Gabal Al-Gharby, Libya, screening the cold-active lipolytic activity of the isolated fungi and selection of the highest cold-active lipase producers. 31 fungal species belong to 12 genera were isolated from these samples with total CFUs of 29560. Fusarium was the most common genus at total CFUs of 9020 and comprising 30.51% from all fungi, followed by Aspergillus, that recorded 25.44% from all fungi. Penicillium was ranked third, nine different species were present. A total of 100% of samples were found to have CFUs of 5140 and 17.4% of all fungi. On lipase production agar medium at two temperatures, 10 and 20°C, 102 fungal isolates from 31 species were tested for their lipolytic activity. The majority of fungi could produce lipase activity at 20°C, where 98 out of 102 isolates the highest lipase producers was higher at 10°C (25) than at 20°C (16). The most active isolates were Alternaria, Fusarium, and Penicillium. Molecular identification of the most active four isolates was carried out by sequencing their internal transcribed spacer region (ITS).

 Keyword: Olive oil, cold active enzymes, Lipase, fungi, lipolytic activity, Fatura.